Document Details

Document Type : Article In Journal 
Document Title :
A short route of covalent biofunctionaliztion of silicon surfaces
طريقة مختصرة للتوظيف الحيوي لأسطح السيليكون
 
Subject : Surface Chemistry 
Document Language : English 
Abstract : Covalently attached organic monolayers on etched Si(1 1 1) surfaces were prepared by heating solutions of 1-alkenes and 1-alkynes in a refluxing mesitylene. Surface modification was monitored by measurement of the static water contact angle, X-ray photoelectron spectroscopy (XPS), infrared reflection absorption spectroscopy (IRRAS), and atomic force microscopy (AFM). Flat and clean N-hydroxysuccinimide (NHS)-ester-terminated/1-decyl mixed monolayers were covalently attached in one step onto a silicon surface. This procedure allows a mild and rapid functionalization of the surface by substitution of the NHS-ester moieties with amines at room temperature. The NHS-ester groups were shown to be fully intact onto the surface. The surface reactivity of the NHS-ester moieties toward amines was qualitatively and quantitatively evaluated via the reaction with methoxytetraethyleneglycolamine (TEGamine) and finally functionalized with single strand and complete DNA molecules. Moreover, domains of DNA were selectively immobilized, on silicon surface making use of TEGamine, which acts as protein repelling agent and therefore prevented non-specific DNA adsorption. The resulting DNA-modified surfaces have shown excellent specificity, and chemical and thermal stability under hybridization conditions. 
ISSN : 09254005 
Journal Name : Sensors and Actuators, B: Chemical 
Volume : 1 
Issue Number : 1 
Publishing Year : 1432 AH
2011 AD 
Article Type : Article 
Added Date : Saturday, January 15, 2011 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
احمد عرفات خميسKhamis, Ahmed ArafatInvestigatorDoctorateakhamis@kau.edu.sa
محمد عبد الرحمن داعوسDaous, Muhammad Abdul RahmanResearcherDoctoratemdaous@kau.edu.sa

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