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Document Type |
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Thesis |
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Document Title |
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COMPARISON OF MIC VALUES MEASURED BY VITEK 2 SYSTEM AND E- TEST METHOD FOR STAPHYLOCOCCUS AUREUS ISOLATES المقارنة بين قيم التركيز المثبط الأدنى (MIC) المقاسة بنظام VITEK 2 وطريقة E-test لعزلات المكورات العنقودية الذهبية |
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Subject |
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Faculty of Applied Medical Sciences > Medical Laboratory Technology Department -MLT |
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Document Language |
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Arabic |
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Abstract |
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Background: One of the major clinical concerns in recent years is the emergence of new strains of Staphylococcus aureus that exhibit reduced susceptibility to glycopeptides, particularly vancomycin. A significant variation was observed in the determination and reporting of MIC results. Although the reported MIC falls within susceptible range, poor outcome and therapeutic failure can be encountered. Aim: This study aimed to compare MIC values of S. aureus isolates that were measured by VITEK 2 system and E-test method for vancomycin. Method: A total of 169 of S. aureus clinical isolates were collected during routine work in the Clinical and Molecular Microbiology Laboratories at King Abdulaziz University Hospital (Jeddah) between December 2013 and August 2014. The MIC values were determined by VITEK 2 system using AST-P580 card and by E-test method.
Results: The average vancomycin MIC for the two methods was 0.9 µg/ml for VITEK 2 and 1.3 µg/ml for E-test. The calculated two-tailed P-value between the two methods was <0.0001 which is considered extremely significant. However, no significant differences were detected in the vancomycin MIC results between MRSA and MSSA sub-populations. Furthermore, the sensitivity results for various antimicrobial agents for these isolates showed that the highest resistance was against penicillin (93%), erythromycin (33%), clindamycin and tetracycline (28%), sulfamethoxazole (27%), gentamicin (22%), and mupirocin (15%). No resistance was detected against teicoplanin, linezolid, and vancomycin.
Conclusion: This study reports a significant difference of MIC measurements between VITEK 2 and E-test, and therefore, it should be emphasized that all vancomycin results obtained for clinical isolates must be confirmed by E-test method to avoid therapeutic failure. |
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Supervisor |
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Dr. Khalil Khalid Alkuwaity |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1437 AH
2016 AD |
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Added Date |
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Wednesday, June 22, 2016 |
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Researchers
| اسراء محمد باصفار | BASAFFAR, ESRAA MOHAMMED | Researcher | Master | |
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