|
Document Type |
: |
Thesis |
|
Document Title |
: |
Induction of Growth Arrest and Apoptosis in Human Breast Cancer Cells by Saudi Propolis Extracts: in vitro Study دراسة معملية لتثبيط النمو وتحفيز الموت المبرمج في نموذج من خلايا سرطان الثدي البشرية باستخدام مستخلص العُكْبر السعودي |
|
Subject |
: |
Faculty of Home Economics |
|
Document Language |
: |
Arabic |
|
Abstract |
: |
Aim: Worldwide, breast cancer continues to be a chronic medical problem, being the most common type of cancer and the leading cause of death in women. Therefore, the development of relatively safe treatments is necessary to reduce the growth of malignant cells and minimize the side effects associated with conventional therapies. The present study was carried out to investigate the ability of a crude ethanolic extract of Saudi Propolis (EESP) to induce growth arrest and apoptosis in human breast cancer cells.
Methods: EESP constituents were analyzed using High Performance Liquid Chromatography (HPLC). The total phenolic and flavonoid content as well as DPPH radical scavenging activity of the extract were determined. Breast carcinoma cell lines; MCF-7, MDA-MB-231, and Normal oral gingival epithelial MOE-1 cells were exposed to increasing doses of EESP for 24, 48 and 72 hours, then cellular viability was evaluated using MTT assay. Colony formation assay was also used to further analyze the cytotoxic effect of EESP. The morphological features of apoptosis were detected using fluorescent stains; Hoechst 33342 and acridine orange/ethidium bromide. Furthermore, DNA laddering, Comet assay, cellular levels of reactive oxygen species (ROS), and mitochondrial membrane potential (ΔΨM) were employed to investigate the pro-apoptotic effects of EESP.
Results: HPLC analyses revealed a significant presence of phenolic acids including Rosmarinic, Cinnamic, Sinapic, Gallic, and Caffeic acids; while the only flavonoid detected was chrysin. EESP exhibited a time-and dose-dependent cytotoxic effect on breast cancer cell lines; MCF-7 and MDA-MB-231. It significantly increased ROS levels, decreased mitochondrial membrane potential, and induced DNA damage. However, EESP had slight or insignificant cytotoxicity on normal oral gingival epithelial cells MOE-1.
Conclusion: Findings suggest that, Propolis may possess a pro-apoptotic potential that might be that might be exploited for prevention of breast cancers. |
|
Supervisor |
: |
Dr. Etab Saleh Alghamdi |
|
Thesis Type |
: |
Master Thesis |
|
Publishing Year |
: |
1438 AH
2017 AD |
|
Added Date |
: |
Sunday, July 16, 2017 |
|
Researchers
| نوره سعد الشهري | Alshehri, Norah Saad | Researcher | Master | |
|