Document Details

Document Type : Thesis 
Document Title :
POTENTIAL EFFECT OF ELETTERIA CARDAMOM EXTRACT ON ENHANCEMENT OF SORFENIB- INDUCED APOPTOSIS IN HEPG2 CELLS.
التأثير المحتمل لمستخلص الهيل في تعزيز موت الخلايا المبرمج الناتج عن السورفينيب في خلايا HepG2.
 
Subject : Faculty of Home Economics 
Document Language : Arabic 
Abstract : The prognosis for liver cancer is very poor; globally it is the second most common cause of death. It can treat with sorafenib, but its use is limited due to its toxicity and adverse reactions. One approach to overcome this toxicity is to use lower doses of sorafenib in combination with other complementary agents. Cardamom seeds are one of the most common ingredients of Indian and Chinese traditional medicine, and different studies have suggested that cardamom extract can display anti-cancer activities. Aim: this study aimed to investigate the efficiency of Elettaria Cardamom Aqueous Extract (ECE) on enhancement of Sorafenib-induced apoptosis in HepG2. Methods: Human liver cancer cells (HepG2) were subjected to increasing doses of single and combined treatments of Sorafenib and ECE. Cells were then harvested after 24 h and cell viability was examined by MTT Assay. Clonogenicity assays and Cell Migration Assay were also carried out. Reactive oxygen species (ROS) generation and mitochondrial membrane potential (MMP) level were determined by DCFH-DA and JC-1 dye, respectively. Finally, agarose gel electrophoresis and comet assays were performed to assess the DNA damage. Result: Combined treatment of ECE with Sorafenib suppressed the proliferation, colony formation and cell migration of HepG2 cells more than the Sorafenib did only. the half maximal inhibitory concentration (IC50), after 24h of incubation were 15 µM of Sorafenib and 9 and 7.3 µM of Sorafenib enhanced by 5 and 10 µg / 100 µl of ECE respectively. HepG2 treated cells displayed biochemical features of apoptotic cell death. The combined treatment increased the ROS production, reduced the level of MMP, increased Comet tail length and induced Deoxyribonucleic acid (DNA) fragmentation more than Sorafenib did alone. Conclusion: These findings demonstrate that ECE enhanced the sorafenib effect in HepG2 cells and suggest that the ECE may be a promising agent for reducing sorafenib side effects in HCC. 
Supervisor : Prof. Amani Aliwi Al-rasheedi 
Thesis Type : Master Thesis 
Publishing Year : 1440 AH
2019 AD 
Added Date : Thursday, July 11, 2019 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
حياة أحمد الغامديAl-Ghamdi, Hayat AhmadResearcherMaster 

Files

File NameTypeDescription
 44698.pdf pdf 

Back To Researches Page