Document Details
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Document Type |
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Thesis |
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Document Title |
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"PROTECTIVE EFFECT OF QUERCETIN AND LUTEOLIN SUPPLEMENTS ON NICOTINE INDUCED OXIDATIVE STRESS AND DNA DAMAGE IN YOUNG RATS" " التأثير الوقائي للمكملات الغذائية الكويرسيتين والليوتيولين على الجهد التأكسدي وتلف الحمض النووي الناجم عن التعرض للنيكوتين في صغار الفئران" |
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Subject |
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Faculty of Home Economics |
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Document Language |
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Arabic |
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Abstract |
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Tobacco consumption is a major risk factor for morbidity and mortality particularly for children. Yearly about 150,000 – 300,000 children younger than 18 month old are exposed to negative smoking. Now, Saudi Arabia imports 20,000 million cigarettes per year, which is the 8th in using tobacco worldwide. This study aimed to investigate the protective effect of Quercetin (Qu) and Luteolin (Lu) on nicotine induced oxidative stress and DNA damage in young rats. Fifty young male Wistar rats weighing 70-80 g rats were divided into five groups (n=10). Group(1): normal control (Cont), Group(2): received nicotine (Ni) (0.75 mg/kg b.w./d) via intra-peritoneal (i.p.), group (3) received nicotine and Quercetin (Ni+Qu) (50mg/kg/d) by gavage, (4) received nicotine and Luteolin (Ni+Lu) (50mg/kg/d), and (5) received nicotine and combination of Qu and Lu (Ni+Qu+Lu) (50mg/kg/d for each). The rats weight recorded weekly for 8 weeks, ended by sacrificing them, serum samples were collected for lipid peroxidation test, liver enzymes and antioxidant enzymes activity. Liver and lungs were weight and subsequently used for DNA fragmentation test and histological examination as biomarkers for toxicological investigations. Results showed significant decreases (p<0.001) in all body and organ weight parameters, significant increases (p<0.05 ) on all liver function enzymes, and lipid peroxidation, while there were significant decreases in antioxidant enzymes and reduced glutathione in Ni group. The study detected marked improvements of all previous parameters in Ni+Lu and Ni+Qu+Lu groups, while the most enhancement of liver enzymes activity were in Ni+Qu group. In the cellular level, the results showed no DNA fragmentation in Qu+Lu , combined group concomitantly with nicotine administration. Histological examinations in liver and lungs tissue showed notable morphological alterations in Ni rat group with marked protection in all supplemented groups. The present finding prove that Luteolin supplement shows the most protective effect against nicotine induce oxidative stress and DNA damage in young male rats.
Key words: Nicotine, flavonoids supplements, oxidative stress, DNA damage |
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Supervisor |
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DR. Sahar A. Abdelaziz |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1441 AH
2019 AD |
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Added Date |
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Thursday, September 19, 2019 |
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Researchers
| وجدان عبدالرحمن بافقيه | Bafageeh, Wejdan Abdulrahman | Researcher | Master | |
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